![]() As such, I believe all cell biologists should be familiar and experienced with this technique. ![]() In the fields of cell biology and electron microscopy, stereology allows scientists to estimate the volume, surface area, number and the size of cellular compartments by looking at a relatively small number of 2D slices and, unlike 3D reconstruction and segmentation (methods which are notoriously slow) it allows scientists to attain these estimates very very fast. The 3D volume of any object can be determined from the 2D areas of its planar sections. Put another way, stereology exploits the fact that many 3D quantities can be determined without 3D reconstruction. To achieve this, stereology uses a random sampling and systematic approach to provide unbiased quantitative data. ![]() Stereology - " the spatial interpretation of sections" - is a technique for 3D interpretation of (2D) planar sections of materials or tissues.
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